CRISPR-Cas: A versatile and scalable gene perturbation system for Genetic Screens

Course Description

Discover the power of CRISPR-Cas, a revolutionary system reshaping genome engineering. This innovative method has transformed research, offering new possibilities for both basic and applied science. CRISPR-Cas stands out for its exceptional scalability, which has revolutionized genetic manipulation.

In collaboration with the Genome Engineering and Measurement Lab (GEML), FGCZ introduces a three-day immersive course tailored to researchers seeking practical expertise in harnessing CRISPR Screens.

Course Highlights:
• Dive into genetic fundamentals: Delve into the theoretical underpinnings of genome-wide functional exploration.

• Pooled genetic screening: Understand the concept of simultaneously assessing thousands of genetic perturbations.

• Design with precision: Learn to craft CRISPR Screens effectively, integrating them with modern NGS techniques.

• From theory to practice: Engage in NGS library preparation, sequencing, and the evaluation of CRISPR libraries.

• Decode data: Gain essential skills in bioinformatic analysis of CRISPR Screen datasets.

By completing this course, participants will achieve the following learning outcomes:

• Differentiate between forward and reverse genetics strategies for genetic exploration.

• Articulate the concept and purpose of a pooled genetic screen.

• Identify key challenges inherent in devising a successful CRISPR screen.

• Calculate representation levels at distinct stages of the CRISPR Screening process.

• Demonstrate competence in generating an NGS library for CRISPR library quality control.

• Evaluate and interpret NGS data obtained from CRISPR library quality control.

• Utilize appropriate software for foundational analysis of CRISPR screen data.

Important facts and how to apply

Target audience and prerequisites

The course is designed for researchers of diverse scientific backgrounds and experience levels; best suited for postdoctoral fellows and graduate students. Good knowledge of standard molecular biology techniques and concepts is advised. Basic understanding of bioinformatic tools is required.

Cost

The course tuition fees for Ph.D. students and post-​docs from the University of Zürich and ETHZ is CHF 200.00 (Swiss francs). For external academics (non-​UZH and non-​ETHZ) the cost is CHF 800.00 per person. For industrial delegates the cost is CHF 1000.00 per person.

IMPORTANT: The internal prices can be applied only, when paid by internal account.

Cancellation policy

Please note that due to the high demand for this course any change related to your participation should be informed as soon as possible, but not later than ten (10) working days prior to the course start date. The notice must be e-​mailed to .

If the cancellation of the course occurs after this time period, the tuition fee will not be refunded.

Please do not hesitate to contact us should you need any additional information or encounter any problems.