Spatial Proteomics Using Mass Spectrometry

Course Description

Spatial proteomics methods are used for studying the organization and the function of proteins in their local micro-environment. Most imaging approaches can determine the spatial distribution of proteins at single-cell resolution, typically focusing on a small number of proteins but lacking the throughput necessary to achieve comprehensive proteome quantification.

The objective of this course is to introduce mass spectrometry (MS)-based spatial proteomics, employing liquid-chromatography (LC) or matrix-assisted laser desorption/ionization (MALDI) imaging MS methods.

The participants will learn how the field evolved from the analysis of entire tissues to the combination of laser-assisted microdissection and LC-MS proteomics for the simultaneous quantification of hundreds to thousands of proteins in specific regions of interest. Moreover, they will become aware of the potential of using MALDI-MS imaging approaches to integrate the spatial analysis of target proteins and other classes of biomolecules (i.e. glycans, small molecules).

The course is designed to bring theory and practice together and introduce the participants to basics MS-based spatial proteomics workflows, including sample preparation, state-of-the-art mass spectrometry, and software for data analysis.

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